DNA

Part:BBa_K4597001:Design

Designed by: Dylan Brettingham   Group: iGEM23_Guelph   (2023-09-16)


E.coli GIF - optomized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The main design consideration was the optimization of the codons in the gene sequence to increase expression in the E. coli chassis we are using.


Source

This part comes from the human genomic sequence but has had some bases changed to optimize the codons for expression in E. coli. IDT's codon optimization tool was used for this.

References